Determination of retinyl esters and retinol in serum or plasma by normal-phase liquid chromatography: Method and applications

54Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.

Abstract

Retinol and retinyl esters are measured in serum or plasma samples by gradient, normal-phase, adsorption 'high-performance' liquid chromatography, with ultraviolet detection at 325 nm. The four major circulating retinyl esters in humans (esters of palmitate, stearate, oleate, and linoleate) are co-eluted as a single peak. Retinyl acetate is included as an internal standard, to correct for variable recovery. Retinol values so measured correlated well (r = 0.88) with those by a widely used reversed-phase chromatographic technique (Clin Chem 1983;29:708-12). The mean retinol concentration was 570 (SEM 17) μg/L and the mean for retinyl esters was 33 (SEM 4) μg/L as determined in samples from 88 fasting young adults. Concentrations of retinol in plasma as low as 50 μg/L can be detected in 100-μL samples, as can 10 μg of retinyl esters per liter. Using this method, we measured absorption of low doses of vitamin A, which may provide a more physiological approach to assessment of fat malabsorption. Additionally, the procedure proved useful for quickly screening for vitamin A toxicity. Major advantages include small sample size, direct injection of the extracted sample without evaporation, rapid elution pattern, co-elution of major retinyl esters as a single peak, and low limit of detection.

Cite

CITATION STYLE

APA

Bankson, D. D., Russell, R. M., & Sadowski, J. A. (1986). Determination of retinyl esters and retinol in serum or plasma by normal-phase liquid chromatography: Method and applications. Clinical Chemistry, 32(1), 35–40. https://doi.org/10.1093/clinchem/32.1.35

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free