Omega-3 fatty acids decrease the proliferation of Rhabdomyosarcoma (RD) and Vero Cell Lines

Abstract

The effect of omega-3 fatty acids, EPA and DHA, and combinations thereof on the viability of Rhabdomyosarcoma (RD) and Vero Cell Lineswas investigated. Various concentrations of these chemicals ranging from 0 to 80 μM were tested using 96-well microtitration plates. Each well contained 0.2 ml of treated cell suspension containing 105 cells/ ml. A micro-ELISA reader was used to measure the changes in cell viability. All tested individual concentrations and combinations reduced viability and this reduction was concentration dependent. Higher values of reduction was seen at 40 μM followed by lesser inhibition at higher concentrations. Data were compared using Student T-test (P value of ≤ 0.05). Mechanisms of effect of these drugs and their combinations are discussed. The present data could be used as a base line for further in vivo investigations and possible clinical considerations. © 2013 Maleek MI.