Transplantation of neural progenitor cells within hyaluronic acid hydrogel in traumatic brain injury in experiment

Abstract

The aim of the study was to evaluate the possibilities of transplantation of autologous neural progenitor cells from C57BL/6 mouse nasal olfactory lamina propria in the hydrogel based on low-, medium-, and high-molecular weight hyaluronic acid during a reconstructive operation after an open traumatic brain injury (TBI) in experiment. Materials and Methods. A reconstructive therapy was performed on the model of an open traumatic brain injury using the autologous neural progenitor cells from C57BL/6 mouse nasal olfactory lamina propria in a hydrogel based on high, medium, and low molecular hyaluronic acid 7 days after injury. Short-term and long-term impairments of neurological functions and memory due to TBI were assessed using a series of behavioral and cognitive tests (modified neurological severity score (mNSS)), open field test, novel object recognition test and passive avoidance behavior). High-field magnetic resonance tomography was used for visualization of a lesion cavity. Results. Implantation of autologous neural progenitor cells in the hydrogel based on high-molecular weight hyaluronic acid in contrast to low- and medium-molecular weight had a protective effect reducing neurological deficit and restoring motor functions, short-term and long-term memory in the posttraumatic period. The effect of hydrogel molecular weight on the morphological characteristics of the injury cavity was less effective during the autologous neural progenitor cells transplantation after TBI. Conclusion. Transplantation of autologous neural progenitor cells from C57BL/6 mouse nasal olfactory lamina propria in the hydrogel based on high-molecular weight hyaluronic acid into the injury focus after open brain trauma promotes functional recovery of reflex and cognitive behavior of animals in the posttraumatic period.