Effect of Extraction and Fractionation on Antioxidant Activity of Extract and Fraction Crescentia Cujete L Leaves

Abstract

Antioxidant compunds like phenols and flavonoids scavenge free radicals and thus inhibit the oxidative mechanisms that lead to control degenerative and other diseases. The aim of this study was to investigate the antioxidant activity in extracts and fractions of Crescentia cujete leaves. 1,1-difenil-2-pikrilhidrazil (DPPH) assay was performed to measure the free-radical scavenging activity of extracts. The results of the study show that leaves of C. cujete possesses significant free radical scavenging properties. All the ethanol extract, etyl acetat extract and fractions exhibited antioxidant activities, however, ethanol fractions of leaves showing the highest antioxidant activity based on the results of DPPH tests.