Arenibacillus arenosus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from sea sand

Abstract

A Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated CAU 1304 T , was isolated from sea sand. Strain CAU 1304 T grew optimally at pH 8.5 and 30 °C and in the presence of 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1304 T belonged to the family Rhodobacteraceae, and was most closely related to Roseicitreum antarcticum ZS2-28 T (96.54% similarity) and Rhodobacter veldkampii ATCC 35703 T (96.46 %). The major fatty acid was C 18 : 1ω 7c and the respiratory quinone was Q-10. The polar lipids were composed of phosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid. The DNA G+C content was 55.9 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that strain CAU 1304 T represents a novel species of a new genus of the family Rhodobacteraceae, for which the name Arenibacillus arenosus gen. nov., sp. nov. is proposed. The type strain of Arenibacillus arenosus is CAU 1304 T (=KCTC 42827 T =NBRC 113022 T ).