Acetamiprid residues in male mice and its effect on liver function

Abstract

This study was performed to identify whether acetamiprid could residue in liver and kidney and its effect on liver function. Fifty adult Kunming male mice weighting 25-30 g were randomly grouped and ten mice in each group (five groups: control, blank, acetamiprid alone, acetamiprid+vitamin E, vitamin E alone); all groups were administered for 35 days by intragastric gavage. The residue in the tissues had been detected by the Liquid Chromatography coupled with tandem Mass Spectrometry (LC-MS/MS) and the serum biochemistry parameters were analyzed by an automatic chemistry analyzer. Acetamiprid induced a wide range of nervous signs in mice; the concentration of acetamiprid in liver was higher than that of kidney p<0.05. Furthermore, acetamiprid increased the activity of Alanine Transaminase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP) (p<0.05 for all) but reduced the activity of Total Protein (TP) and Albumin (ALB) (p<0.05, for both). However, Vitamin E ameliorated the effects of acetamiprid on these parameters, compared to the acetamiprid only group (p<0.05). The results indicated that the liver was the main organ of acetamiprid residues and acetamiprid could affect liver function but VE could reduce acetamiprid-induced impairment. © Medwell Journals, 2012.