A gel mobility retardation assay can be used to detect a protein-protein interaction. The assay is based on the electrophoretic mobility of a protein-protein complex being less than that of either protein alone. Electrophoretic mobility is detected by the fluorescence of a green fluorescent protein variant that is fused to one of the protein partners. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study.
CITATION STYLE
Park, S. H., & Raines, R. T. (2004). Fluorescence gel retardation assay to detect protein-protein interactions. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1385/1-59259-762-9:155
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