Estimation of Xyloglucan in Vegetables by Enzyme-Linked Immunosorbent Assay

Abstract

We prepared the antibody specific against plant xyloglucan and applied it to enzyme-linked immunosorbent assay in order to estimate xyloglucan contents in several vegetables. In this experiment, xyloglucan was detected in the 24% aqueous potassium hydroxide-soluble fractions of tomato, cabbage, lettuce, and eggplant. The xyloglucan contents of these vegetables were estimated to be 1.4 mg, 54 fig, 14 ng, and 4 fig per one hundred grams of their edible portions, respectively, using tamarind xyloglucan as a standard xyloglucan. © 1993, Center for Academic Publications Japan. All rights reserved.