Development of Lactate Biosensor based on Electro statically Functionalized Graphene oxide bound Lactate oxidase

Abstract

The search for new advanced materials is an important area of contemporary research in numerous disciplines of science and the development of many new technologies. Great attention has been paid in recent years to nano-structured materials of different chemical composition, produced as nanoparticles, nanowires or nanotubes. In various fields of chemical analysis, there have been an increasing number of applications of graphene based material. In the present work Lactate oxidase has been immobilized onto the surface of graphene oxide polymeric film through glutraldehyde coupling. This film acted as a working electrode. The resulting biosensor was characterized electrochemically and detection performances were evaluated. It can keep more than 80% of its initial activity after continuously using for three months. The sensor is also unaffected by the various serum interfering agent. The present lactate biosensor showed excellent properties for the sensitive determination of lactic acid with good reproducibility and remarkable stability.