The determination of the localization of various compounds in a whole animal is valuable for many applications, including pharmaceutical absorption, distribution, metabolism, and excretion (ADME) studies and biomarker discovery. Imaging mass spectrometry is a powerful tool for localizing compounds of biological interest with molecular specificity and relatively high resolution. Utilizing imaging mass spectrometry for whole-body animal sections offers considerable analytical advantages compared to traditional methods, such as whole-body autoradiography, but the experiment is not straightforward. This chapter addresses the advantages and unique challenges that the application of imaging mass spectrometry to whole-body animal sections entails, including discussions of sample preparation, matrix application, signal normalization, and image generation. Lipid and protein images obtained from whole-body tissue sections of mouse pups are presented along with detailed protocols for the experiments. © 2010 Springer Science+Business Media, LLC.
CITATION STYLE
Reyzer, M. L., Chaurand, P., Angel, P. M., & Caprioli, R. M. (2010). Direct molecular analysis of whole-body animal tissue sections by MALDI imaging mass spectrometry. Methods in Molecular Biology, 656, 285–301. https://doi.org/10.1007/978-1-60761-746-4_17
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