Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Takahashi, D., Li, B., Nakayama, T., Kawamura, Y., & Uemura, M. (2014). Shotgun proteomics of plant plasma membrane and microdomain proteins using Nano-LC-MS/MS. Methods in Molecular Biology, 1072, 481–498. https://doi.org/10.1007/978-1-62703-631-3_33
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