The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system. [Figure not available: see fulltext.].
CITATION STYLE
Wu, X., Kriz, A. J., & Sharp, P. A. (2014, June 1). Target specificity of the CRISPR-Cas9 system. Quantitative Biology. Higher Education Press. https://doi.org/10.1007/s40484-014-0030-x
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