This chapter describes immunohistochemistry on Drosophila heads (brains) with respect to antigens involved in the circadian system. Two different methods have been successfully performed in several labs: immunolabeling on whole-mount brains and on cryostat sections of entire heads. Both methods are addressed here. The primary antisera can be detected by enzyme-labeled or fluorescence-labeled secondary antibodies. The advantages of the different methods are discussed.
CITATION STYLE
Helfrich-Förster, C. (2007). Immunohistochemistry in Drosophila (pp. 533–547). https://doi.org/10.1007/978-1-59745-257-1_42
Mendeley helps you to discover research relevant for your work.