This is a standard protocol for the detection of intracellular proteins by indirect immunofluorescence microscopy in DT40. It has been used extensively to investigate the intracellular distribution of various proteins of the endocytic machinery.
CITATION STYLE
Wettey, F. R., & Jackson, A. P. (2006). Indirect immunofluorescence microscopy. Sub-Cellular Biochemistry, 40, 427–429. https://doi.org/10.1007/978-1-4020-4896-8_40
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