Development and Validation of UV Spectrophotometric Method for the Estimation of Fluconazole in the Marketed Dosage Formulations

Abstract

The aim of current study was to develop and validate UV Spectrophotometric method for Fluconazole. Ultra Violet Spectroscopy was carried out at 260nm and samples were prepared with a solution of phosphate buffer pH 7.4. The linearity demonstrated a correlation coefficient of 0.998. The method was subjected to validity for the parameters as per ICH guidelines. Correctness, exactness, LOD, LOQ, recovery study and range were determined and the parameters were determined by performing the repeated experiments and proper sampling of the solution. The objective of selection of UV spectroscopy was the simplicity, time and economy of the method furthermore the sensitivity was also high by UV spectroscopy as HPLC technique is costly, time consuming and number of factors may affect the determination. It was observed that, the proposed method was linear, correct, repeatable, error free, selective, specific and cost effective proving the dependability of the method. More over same solvent was used throughout the experimental work and it was found that the method was free from any type of interference from any excipients and method was simple, rapid, precise, accurate and sensitive and can be applied in routine analysis of Fluconazole in single and combined form.