Establishing sw1353 chondrocytes as a cellular model of chondrolysis

Abstract

Abstract: Osteoarthritis (OA) is the most common degenerative joint disease characterised by chon- drocyte cell death. An in vitro model of chondrocyte cell death may facilitate drug discovery in OA management. In this study, the cytotoxicity and mode of cell death of SVV1353 chondrocytes treated with 24 h of OA inducers, including interleukin-l|S (IL-1|S), hydrogen peroxide (HoOj) and monosodium iodoacetate (MIA), were investigated. The microscopic features, oxidative (iso- prostane) and inflammatory markers (tumour necrosis factor-α TNF-a) for control and treated cells were compared. Our results showed that 24 h of HjOj and MIA caused oxidative stress and a concentration-dependent reduction of SW1353 cell viability without TNF-a level upregulation HjOj primarily induced chondrocyte apoptosis with the detection of blebbing formation, cell shrinkage and cellular debris. MIA induced S-phase arrest on chondrocytes with a reduced number of attached cells but without significant cell death. On the other hand, 24 hof IL-1|S did not affect the cell morphology and viability of SVV1353 cells, with a significant increase in intracellular TNF-a levels without induc- ing oxidative stress. In conclusion, each OA inducer everts differential effects on SW1353 chondrocyte cell fate. IL-13 is suitable in the inflammatory study but not for chondrocyte cell death. FkOj and MIA are suitable for inducing chondrocyte cell death and growth arrest, respectively.