Cultivation of Vero Cells on Microporous and Macroporous Microcarriers

Abstract

The manufacture of immunobiologicals, such as viral vaccines, is largely based on animal cell technology. Large-scale production of viral antigens depends on efficient cell proliferation and virus replication, both in terms of quality and quantity. This can be achieved by choosing adequate culture conditions and cultivation systems. Microcarriers are a tool to provide a large adhesion area in homogeneous bioreactor systems, allowing high densities of anchorage-dependent cells to be obtained. The aim of the present work was to study the use of different microcarriers and culture conditions to obtain highdensity Vero cell cultures for the production of viral antigens.