Intracranial AAV-IFN-b gene therapy eliminates invasive xenograft glioblastoma and improves survival in orthotopic syngeneic murine model

Abstract

The highly invasive property of glioblastoma (GBM) cells and genetic heterogeneity are largely responsible for tumor recurrence after the current stan-dard-of-care treatment and thus a direct cause of death. Previously, we have shown that intracranial interferon-beta (IFN-b) gene therapy by locally administered adeno-associated viral vectors (AAV) successfully treats nonin-vasive orthotopic glioblastoma models. Here, we extend these findings by testing this approach in invasive human GBM xenograft and syngeneic mouse models. First, we show that a single intracranial injection of AAV encoding human IFN-b eliminates invasive human GBM8 tumors and promotes long-term survival. Next, we screened five AAV-IFN-b vectors with different promoters to drive safe expression of mouse IFN-b in the brain in the context of syngeneic GL261 tumors. Two AAV-IFN-b vectors were excluded due to safety concerns, but therapeutic studies with the other three vectors showed extensive tumor cell death, activation of microglia surround-ing the tumors, and a 56% increase in median survival of the animals treated with AAV/P2-Int-mIFN-b vector. We also assessed the therapeutic effect of combining AAV-IFN-b therapy with temozolomide (TMZ). As TMZ affects DNA replication, an event that is crucial for second-strand DNA synthesis of single-stranded AAV vectors before active transcription, we tested two TMZ treatment regimens. Treatment with TMZ prior to AAV-IFN-b abro-gated any benefit from the latter, while the reverse order of treatment dou-bled the median survival compared to controls. These studies demonstrate the therapeutic potential of intracranial AAV-IFN-b therapy in a highly migratory GBM model as well as in a syngeneic mouse model and that combination with TMZ is likely to enhance its antitumor potency.