A web tool to discover full-length sequences - Full-lengther

Abstract

Many Expressed Sequence Tags (EST) sequencing projects produce thousands of sequences that must be cleaned and annotated. This research presents the so-called Full-Lengther, an algorithm that can find out full-length cDNA sequences from EST data. To accomplish this task, Full-Lenther is based on a BLAST report using a protein database such as UniProt. Blast alignments will guide to locate protein coding regions, mainly the start codon. Full-Lengther contains an ORF prediction algorithm for those cases which is not homologous to any sequence. The algorithm is implemented as a web tool to simplify its use and portability. This can be worldwide accessible via http://castanea.ac.uma.es/genuma/ full-lengther/. © 2007 Springer-Verlag Berlin Heidelberg.