Efeito dos aminoácidos de cadeia ramificada no músculo imobilizado e no músculo desnervado

Abstract

Objective: This study aimed to histologically evaluate the soleus muscle in a state of immobilization and denervation in rats supplemented with branched chain amino acids. Materials and Methods: To conduct the study 30 male rats navergicus Albinus, Wistar variety, were used, with 3-4 months of age, divided into 6 groups (n=5): control (C), denervated 7 days (D), immobilized 7 days (I), supplemented with branched chain amino acids 7 days (BCAA), denervated supplemented with branched chain amino acids (BCAA + D) and immobilized supplemented with branched chain amino acids (BCAA + I). The animals were denervated by sectioning the sciatic nerve (1 cm). The immobilized groups received a stent coupled with acrylic resin in the hind paw promoting immobilization. After the experimental period the animals were euthanized and the muscles were analyzed histologically. The Shapiro-Wilk test evaluated the normality of the data, followed by analysis of variance and then Tukey- Kramer with significance level below 5%. Results: Supplementation increased the cross-sectional area of the muscle fiber in 74.94% in group I+BCAA and 90.37% in group D+BCAA compared to groups I and D, respectively. There was an increase of 97.21% in the connective tissue area density in the BCAA group compared to group C, although supplementation promoted reduction of 34.44% in group D+BCAA compared to group D, however, there was no significant difference between groups I and BCAA+I. Conclusions: It is observed that the use of branched chain amino acids can decrease the muscle atrophy caused by disuse, both the condition as provided by denervation or immobilization. (English) [ABSTRACT FROM AUTHOR]