PCR detection and 16S rDNA sequence analysis of different Acidithiobacillus ferrooxidans isolates

Abstract

Acidithiobacillus ferrooxidans is one of the most important microorganisms involved in bioleaching, acid mine drainage and biodesulphurization of coals. In this study A. ferrooxidans is detected in liquid samples using specific primers for 16S rDNA PCR. The applied PCR technique is accomplished using template DNA from pure cultures of the microorganisms. PCR amplification products were subjected to bioinformatical analysis. The results obtained from these analyses are a good foundation for further molecular experiments with different A. ferrooxidans isolates.