Biomimetic Sequestration of CO2 Using Carbonic Anhydrase from Calcite Encrust Forming Marine Actinomycetes

Abstract

See, stats, and : https : / / www . researchgate . net / publication / 273126558 Biomimetic Anhydrase Actinomycetes ARTICLE DOI : 10 . 17311 / sciintl . 2015 . 48 . 57 READS 63 5 , INCLUDING : Pravin Veer 40 SEE Chintan Veer 6 SEE Shakuntla 1 SEERajeshHemchandracharya60SEEAvailable:ChintanRetrieved:27ABSTRACTBackground:CO2intheenvironmenthasbeenaburningissueandaggravatedthethreatsofglobalwarming.VariousstrategiesarebeingusedtodecreasethelevelofreleasingCO2.Biosequestrationisaoneoftheavailablenontoxic,robustandeco-friendlyapproachesbutstilllessevaluated.ThisstudyreportstheroleofmicrobialCarbonicAnhydrase(CA)intheformationofcalcitebyutilizingCO2.Methods:ThemarineNocardiopsislucentensiswasstudiedforintracellularmicrobialCA.Isolategrownoptimallyat2.5%CO2saturatedenvironmentandprocedurethecalciumcarbonateencrustinthepresenceofCaCl2.Results:TheenzymewasfoundtocatalyzethereactioninawiderangeofpHandtemperaturewithanoptimumat7.0pHand25°C.The50mMNaCl,KClandMgCl2werefoundtosupporttheenzymeactivityand50mMZnCl2increasesactivity1.5fold.CAwasabletowithstandagainstsulphanilamideinhibitorandstableoverprolongedincubationat4and37°C.Conclusion:Calciteformationwasevaluatedwithandwithoutenzymeusingmarinewaterasasourceofcalciumions.TheresultofSEMandEDXindicatedtheformationoflargerflower-shapedparticlescomparetothesmallcubicparticlesformedwithoutenzyme.TheresultssuggestthattherobustnessofenzymeandsuitabilityintheCO2capturereactor.