Anti-inflammatory and detoxification activities of some Ipomoea species determined by ion channel inhibition and their phytochemical constituents

Abstract

To get more scientific information to support the medicinal uses of Ipomoea batatas, I. carnea, and I. pes-caprae, phytochemical constituent analysis, toxicity and detoxification testing on normal and poisoned human cells, and ion channel inhibition using VGSCs, VGKCs and hERG channel were investigated. GC-MS and HPLC analysis were used for phytochemical screening and measuring the major compounds in 100 g leaf samples, which were 53.15-1446.93 mg oleamide in I. batatas - I. pes-caprae, 1.51-59.57 mg phytol in I. batatas - I. pes-caprae, and 39.04 mg α-amyrin and 8.09 mg β-amyrin in I. pes-caprae. The MTT assay showed no IC50 value, and the comet assay revealed insignificant DNA damages (p > 0.05) on normal PBMCs. Detoxification efficacy for rice whisky- and herbicide-exposed cells was tested, and higher cell viability percentages were shown when the poisoned cells were treated with I. carnea and I. pes-caprae extracts compared with negative control. The hexane and acetonitrile extracts of I. batatas, I. carnea, and I. pes-caprae inhibited the VGSCs (Nav1.6 and 1.8) and VGKCs (Kv1.1 and Kv10.1) channels, but not the hERG channel. The overall results indicated that the pharmacological activities of the studied plant extracts allowed them to be used as an excellent analgesic, an early marker in tumor formation useful for diagnosis and therapy of tumors, and treatments of poison and drug addiction, in addition to their antimicrobial, anti-inflammatory, and antipyretic activities. The plants can be used in daily human life with good cardiac safety and low risk for hERG inhibition.