The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens

23Citations
Citations of this article
51Readers
Mendeley users who have this article in their library.

Abstract

The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive samples for at least one of the three etiologic agents when soil was supplemented with limestone. The results expose some critical contamination points, providing support for training farmers on good management practices during the production process.

Cite

CITATION STYLE

APA

Ferreira, F. P., Caldart, E. T., Freire, R. L., Mitsuka-Breganó, R., de Freitas, F. M., Miura, A. C., … Navarro, I. T. (2018). The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens. Revista Brasileira de Parasitologia Veterinaria, 27(3), 327–337. https://doi.org/10.1590/s1984-296120180050

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free