Culture and maintenance of human embryonic stem cells: A potential source for vasculargenesis

Abstract

Regenerative and cell-replacement therapies remain one of the major goals in biomedicine. With an increasing aging population coupled with a corresponding increase in cardiovascular pathologies, the requirement for cell-based therapies and vascular regenerative medicine is more pressing. Human Embryonic Stem cells present a source for regenerative therapies in vascular medicine. Therefore, careful culture and maintenance of these stem cells is imperative. This chapter will outline the procedures for the culture and maintenance of Human Embryonic Stem cells. These cells can then be utilised for vascular regeneration.