Proteomic analysis of plasma of patients with left ventricular remodeling after myocardial infarction: Usefulness of SELDI-TOF

Abstract

SELDI-TOF and depletion of major blood proteins is one of the most promising approaches for accessing low-abundance biomarkers. The use of combinatorial peptide ligand library (CPLL) for selecting the lowabundance proteins and of liquid-phase isoelectric focusing for purifying the corresponding proteins was tested in plasma or serum from patients with myocardial infarction (MI). Here, we describe the SELDI pro fi ling of CPLL-treated plasma to select low-abundance proteins in plasma and the strategy for puri fi cation and mass spectrometry identi fi cation. This approach shows the potential to select and identify candidate biomarkers in patients with left ventricular remodeling after MI. © 2013 Springer Science+Business Media New York.