The detection of Haemophilus influenzae by conventional methods like culture is time-consuming and may give false-negative results, especially during ongoing antibiotic treatment. Therefore, non-culture based methods that are sensitive, specific, and rapid are valuable for early diagnosis and effective therapy. Here we describe a quantitative real-time PCR assay based on the outer membrane P6 gene omp6, to detect H. influenzae and its application on respiratory tract specimens. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Abdeldaim, G. M. K., & Herrmann, B. (2013). PCR detection of haemophilus influenzae from respiratory specimens. Methods in Molecular Biology, 943, 115–123. https://doi.org/10.1007/978-1-60327-353-4_7
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