Predictive value of long non-coding RNA intersectin 1-2 for occurrence and in-hospital mortality of severe acute pancreatitis

Abstract

Background: This study aimed to investigate the predictive value of long non-coding RNA intersectin 1-2 (lnc-ITSN1-2) for severe acute pancreatitis (SAP) risk, and its correlation with disease severity and in-hospital mortality in SAP patients. Methods: Plasma samples from 60 SAP, 60 moderate-severe acute pancreatitis (MSAP) and 60 mild acute pancreatitis (MAP) patients were collected within 24 hours, and plasma samples from 60 age and gender-matched healthy controls (HCs) were collected when enrollment. Lnc-ITSN1-2 was detected by reverse transcription-quantitative polymerase chain reaction. In AP patients, disease severity was evaluated and in-hospital deaths were recorded. Results: Lnc-ITSN1-2 was increased in SAP patients compared with MSAP, MAP patients, and HCs, and it is well-discriminated SAP patients from MSAP patients (area under curve (AUC): 0.699, 95% confidence interval (CI): 0.605-0.792), MAP patients (AUC: 0.862, 95% CI: 0.798-0.926), and HCs (AUC: 0.958, 95% CI: 0.925-0.990). For disease severity, lnc-ITSN1-2 was positively correlated with Ranson's score, acute pathologic and chronic health evaluation (APACHE) II score, sequential organ failure assessment (SOFA) score, and C-reactive protein (CRP) in SAP patients, MSAP patients, and MAP patients; meanwhile, the correlation coefficients were highest in SAP patients. Furthermore, lnc-ITSN1-2 displayed a good predictive value for increased in-hospital mortality in SAP (AUC: 0.803, 95% CI: 0.673-0.933) and MSAP (AUC: 0.854, 95% CI: 0.752-0.956) patients, which was similar with several common prognostic factors (including Ranson's score, APACHE II score, SOFA score, and CRP). Conclusion: Lnc-ITSN1-2 might be a potential biomarker for discrimination of SAP to improve the prognosis of SAP patients.