Ligand‐induced conformational changes of citrate synthase studied with the fluorescent probe 8‐anilinonaphthalene 1‐sulfonate

Abstract

Experiments are presented which show that oxaloacetate and analogs thereof with (R)‐malate substructure, on interaction with citrate synthase linked to synthase 8‐anilinonaphthalene 1‐sulfonate (ANS), induce identical conformational changes of a characteristic magnitude. A conformational change of lower magnitude is also produced on binding of CoASH or ATP to citrate synthase · ANS and is completed on addition of oxaloacetate. The significance of these ligand‐dependent conformational changes is discussed. Copyright © 1989, Wiley Blackwell. All rights reserved