Single-cell analysis of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) provides a means to perform highly detailed kinetic studies, assess heterogeneity between cells, and distinguish the subcellular localization of ERK activity. We describe here the methods needed to perform such measurements in a cell type of the investigator’s choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.
CITATION STYLE
Pargett, M., Gillies, T. E., Teragawa, C. K., Sparta, B., & Albeck, J. G. (2017). Single-cell imaging of ERK signaling using fluorescent biosensors. In Methods in Molecular Biology (Vol. 1636, pp. 35–59). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7154-1_3
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