Lysophosphatidylcholine activates a novel PKD2-Mediated signaling pathway that controls monocyte migration

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Abstract

OBJECTIVE-: Monocyte activation and migration are crucial events in the development of atherosclerosis and other inflammatory diseases. This study examined the role of protein kinase D (PKD) in monocyte migration. METHOD AND RESULTS-: PKD2 is the predominant isoform of PKD expressed in monocytic THP-1 cells and primary human monocytes. Lysophosphatidylcholine (lysoPC), a prominent component of oxidized low-density lipoprotein, induces rapid and marked PKD activation in these cells. Using multiple approaches, including dominant-negative mutants and small interfering RNA knock-down, we found that lysoPC-induced PKD2 activation was required for the activation of both ERK and p38 MAPK. p38 MAPK mediation of lysoPC-induced monocytic cell migration was reported previously; our results reveal that the lysoPC-induced PKD2-p38 pathway controls monocyte migration. CONCLUSIONS-: This study provides the first evidence that (1) lysoPC activates PKD, (2) PKD2 has a novel role in p38 activation, and (3) the PKD2-activated p38 pathway is responsible for lysoPC-induced migration of THP-1 cells and human monocytes. Thus, PKD is a novel and functional intracellular regulator in both lysoPC signaling and monocyte migration. These results suggest a new role for PKD2 in the development of atherosclerosis and other inflammatory diseases. © 2009 American Heart Association, Inc.

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Tan, M., Hao, F., Xu, X., Chisolm, G. M., & Cui, M. Z. (2009). Lysophosphatidylcholine activates a novel PKD2-Mediated signaling pathway that controls monocyte migration. Arteriosclerosis, Thrombosis, and Vascular Biology, 29(9), 1376–1382. https://doi.org/10.1161/ATVBAHA.109.191585

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