Mucosal-associated invariant T (MAIT) cells are a novel subset of innate-like T cells that recognize vitamin B metabolites from a range of microbes presented by MHC class I-related molecules (MR1). The term mucosal-associated invariant T cells derives from the fact that MAIT cells are abundant in the liver and mucosal tissues, and human MAIT cells use a semi-invariant TCR Vα7.2 Jα33 paired with Vβ2 or Vβ13. Here, based on the interaction between MAIT cell and its ligand 5-OP-RU/MR1, we describe the protocols for identification, rapid expansion, and isolation of human MAIT cells.
CITATION STYLE
Liu, Y., Wang, W., Wu, X., & Weng, X. (2020). Detection, Expansion, and Isolation of Human MAIT Cells. In Methods in Molecular Biology (Vol. 2111, pp. 285–293). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0266-9_22
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