A Plant Caspase-Like Protease Activated during the Hypersensitive Response

155Citations
Citations of this article
147Readers
Mendeley users who have this article in their library.
Get full text

Abstract

To test the hypothesis that caspase-like proteases exist and are critically involved in the implementation of programmed cell death (PCD) in plants, a search was undertaken for plant caspases activated during the N gene-mediated hypersensitive response (HR; a form of pathogen-induced PCD in plants) in tobacco plants infected with Tobacco mosaic virus (TMV). For detection, characterization, and partial purification of a tobacco caspase, the Agrobacterium tumefaciens VirD2 protein, shown here to be cleaved specifically at two sites (TATD and GEQD) by human caspase-3, was used as a target. In tobacco leaves, specific proteolytic processing of the ectopically produced VirD2 derivatives at these sites was found to occur early in the course of the HR triggered by TMV. A proteolytic activity capable of specifically cleaving the model substrate at TATD was partially purified from these leaves. A tetrapeptide aldehyde designed and synthesized on the basis of the elucidated plant caspase cleavage site prevented fragmentation of the substrate protein by plant and human caspases in vitro and counteracted TMV-triggered HR in vivo. Therefore, our data provide a characterization of caspase-specific protein fragmentation in apoptotic plant cells, with implications for the importance of such activity in the implementation of plant PCD.

References Powered by Scopus

Cleavage of structural proteins during the assembly of the head of bacteriophage T4

219953Citations
N/AReaders
Get full text

Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics

13678Citations
N/AReaders
Get full text

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

11115Citations
N/AReaders
Get full text

Cited by Powered by Scopus

NO news is good news for plants

401Citations
N/AReaders
Get full text

A cut above the rest: The regulatory function of plant proteases

339Citations
N/AReaders
Get full text

Mechanisms of plant resistance to viruses

288Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Chichkova, N. V., Kim, S. H., Titova, E. S., Kalkum, M., Morozov, V. S., Rubtsov, Y. P., … Vartapetian, A. B. (2004). A Plant Caspase-Like Protease Activated during the Hypersensitive Response. Plant Cell, 16(1), 157–171. https://doi.org/10.1105/tpc.017889

Readers over time

‘09‘10‘11‘12‘13‘14‘15‘16‘17‘18‘19‘20‘21‘22‘23‘2406121824

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 60

55%

Researcher 30

27%

Professor / Associate Prof. 14

13%

Lecturer / Post doc 6

5%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 96

81%

Biochemistry, Genetics and Molecular Bi... 16

14%

Environmental Science 4

3%

Immunology and Microbiology 2

2%

Save time finding and organizing research with Mendeley

Sign up for free
0