The G1/S-phase restriction point is an important landmark in the mammalian cell division cycle. The key regulator of the G1/S transition is the retinoblastoma gene product (pRB). It prevents the transcription of genes required for S-phase progression by repressing E2F transcription factors. An increase in Cdk phosphorylation of pRB causes the release of E2F transcription factors and advancement into S phase. Here we describe two simple techniques used to assess pRB phosphorylation and E2F transcription during G1/S progression.
CITATION STYLE
Thwaites, M. J., Cecchini, M. J., & Dick, F. A. (2014). Analyzing RB and E2F during the G1–S transition. Methods in Molecular Biology, 1170, 449–461. https://doi.org/10.1007/978-1-4939-0888-2_24
Mendeley helps you to discover research relevant for your work.