Evaluation of a [ 67Ga]-thiosemicarbazone complex as tumor imaging agent

Abstract

[ 67Ga]labeled 2-acetylpyridine 4,4-dimethylthiosemicarbazone ([ 67Ga]-[APTSM 2] 2+) was prepared using freshly prepared [ 67Ga]GaCl 3 and 2-acetylpyridine 4,4-dimethylthiosemicarbazone (APTSM 2) for 30 min at 90°C (radiochemical purity: >97% ITLC, >98% HPLC, specific activity: 15-20 Ci/mmol). Stability of the complex was checked in human serum for 37°C. The biodistribution of the labeled compound in vital organs of normal and fibrosarcoma bearing mice were compared with that of free Ga 3+ cation up to 24h. Initial SPECT images and biodistribution results showed significant tumor uptake in fibrosarcoma-bearing mice after 2 hour post injection. © Jalilian et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H.