Phytotoxicity of silver nanoparticles to cucumber (Cucumis sativus) and wheat (Triticum aestivum)

Abstract

The increasing release of silver (Ag) nanoparticles (NPs) into the environment highlights the importance of exploring the interactions between Ag NPs and plants, which are the basis of most ecosystems. In this study, two plant species, Cucumis sativus L. (cucumber) and Triticum aestivum L. (wheat) were exposed to Ag NPs and Ag+ (added as AgNO3) at the germination and vegetative growth stages. Above certain concentrations, Ag NPs and Ag+ were toxic to the two plants. However, stimulatory effects were observed on root elongation for the cucumbers that were exposed to Ag NPs at concentrations below 200 mg/L, and Ag+ at concentrations below 5 mg/L. The two plants were more susceptible to the toxicity of Ag NPs at the vegetative growth stage than the germination stage. Ag was accumulated in the roots and was subsequently translocated to the shoots after the exposure to Ag NPs. To assess the role of released Ag+, we measured the dissolution of Ag NPs in exposure solutions. About 0.03% and 0.01% of Ag NPs were dissolved into a hydroponic solution at the germination stage for cucumber and wheat, respectively; while 0.17% and 0.06% at the vegetative period for cucumber and wheat, respectively. Cysteine, a strong chelating ligand of Ag+, could completely eliminate the effects of Ag NPs on cucumber and wheat, suggesting that the phytotoxicity of Ag NPs was possibly caused by the release of Ag+. © 2014 Zhejiang University and Springer-Verlag Berlin Heidelberg.