The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis. © 2013 by Anais Brasileiros de Dermatologia.
CITATION STYLE
Dantas, K. C., Freitas, R. S., Vicentini Moreira, A. P., da Silva, M. V., Vasconcellos, C., Benard, G., & Criado, P. R. (2013). Uso da Reação em Cadeia da Polimerase aninhada (PCR aninhada) para o diagnóstico precoce da infecção pelo Histoplasma capsulatum no soro e no sangue total de pacientes HIV positivos. Anais Brasileiros de Dermatologia, 88(1), 141–143. https://doi.org/10.1590/S0365-05962013000100025
Mendeley helps you to discover research relevant for your work.