Glucose-based carbon electrode for trace-level detection of acetaminophen

Abstract

A highly sensitive electrochemical method for trace-level detection of acetaminophen (AMP) was employed using glucose-based carbon electrode (Gu/CPE) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Electrochemical properties of AMP were well suited in 0.2 M phosphate buffer solution (PBS) of pH 7.0 and peak intensity of AMP at the Gu/CPE was enhanced compared to the bare carbon paste electrode. Furthermore, the different parameters, such as pH of electrolyte, scan rate, accumulation time effects, were investigated by CV. The redox reaction of AMP was quasi-reversible as controlled by a diffusion process with two protons and two electrons. As per DPV technique, a lower detection limit (LOD) of 3.72 × 10–8 M over a wide linearity range (0.6-9.0 µM) was observed. The excipient study demonstrates the selectivity of the electrode toward the electrochemical detection of AMP. Furthermore, the electrode was utilized to quantify AMP in clinical and pharmaceutical samples.