Antioxidant activity, total phenolic and total flavonoid content of hydroalcoholic extract of Artocarpus lacucha Buch-Ham. Leaves

Abstract

The uncontrolled production of oxygen free radicals and the unbalanced mechanism of antioxidant protection results in the onset of many diseases, such as cancer, diabetes, Alzheimer's, heart diseases and aging. Artocarpus lacucha Buch-Ham. belongs to the family of Moraceae, popularly regarded as a medicinal plant and commonly called as monkey jack. This plant is widely distributed in the tropical regions of south and south-east Asia. The aim of this study was to determine antioxidant activity, total flavonoid and total phenolic content of Artocarpus lacucha Buch-Ham. Leaves. Extract was prepared using ethanol 80% with maceration method. Antioxidant activity were determined with 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Total flavonoid and total phenolic content were determination with colorimetric methods. Antioxidant activity from DPPH assay measured as IC50 was 48.23 ± 0.46 µg/mL. Extract was found to contain high levels of total phenolic (79.87 ± 0.39 mg GAE/g) and total flavonoid (9.65 ± 0.03 mg QE/g). The results reveal that hydroalcoholic extract of Artocarpus lacucha Buch-Ham. Leaves has antioxidant potential.