Ectomycorrhiza formation between Eucalyptus pilularis Sm. and Hydnangium carneum Wallr. in Dietr.

Abstract

Ectomycorrhizas were synthesized in growth pouches between Eucalyptus pilularis Sm. and Hydnangium carneum Wallr. in Dietr. Colonization of lateral roots occurred within 14 d after inoculation with the development of a dense white mantle and a paraepidermal Hartig net. Cystidium‐like structures and unusual hyphal coils were present on the surface of the mature mantle. The mantle consisted of distinctive outer and inner portions with small, uniform hyphae comprising the outer mantle and much enlarged and modified hyphae in the inner mantle. Granular deposits were abundant primarily in the inner mantle hyphae as shown by light and fluorescence microscopy. These granules were shown to be polyphosphate through cytochemical tests and the use of energy dispersive X‐ray microanalysis. Phosphorus and calcium were the main elements identified in these granules. Copyright © 1989, Wiley Blackwell. All rights reserved