Method for Living Cell Mechanical Properties Evaluation from Force-Indentation Curves

Abstract

Living cells mechanical properties establishment from Atomic force microscopy (AFM) force-separation curves is a challenge because the calculated Young’s modulus depends on the applied mathematical model. The more reliable results can be obtained using finite element models. In this work, yeast cells with different mechanical properties were measured by AFM. To change cells mechanical properties, yeasts were immersed in 9, 10-phenanthrenequinone, which changed cells’ membranes elasticity. 3D finite element model of the whole cell was created to calculate reacting force when AFM tip indents the cell in the same way as in the real experiment. It was found that our model is capable to draw the information about cells mechanical properties and visco-elastic behavior of cells membranes.