Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv), which represent the smallest part of an antibody containing the entire antigen-binding site, find increasing usage as targeting moiety. Here we provide protocols for the preparation of type II scFv immunoliposomes by the conventional coupling method as well as the post-insertion method. Furthermore protocols to analyze binding of these immunoliposomes to antigen-expressing cells as well as internalization through receptor-mediated endocytosis are included.
CITATION STYLE
Rothdiener, M., Beuttler, J., Messerschmidt, S. K. E., & Kontermann, R. E. (2010). Antibody targeting of nanoparticles to tumor-specific receptors: immunoliposomes. Methods in Molecular Biology (Clifton, N.J.), 624, 295–308. https://doi.org/10.1007/978-1-60761-609-2_20
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