Abscisic acid is involved in root cell wall phosphorus remobilization independent of nitric oxide and ethylene in rice (Oryza sativa)

Abstract

Background Abscisic acid (ABA) is a well-studied phytohormone demonstrated to be involved in sub-sets of stress responses in plants, such as iron (Fe) deficiency and phosphorus (P) deficiency in Arabidopsis. However, whether ABA is involved in P deficiency in rice has not been frequently studied. The present study was undertaken to investigate the mechanism underlying ABA-aggravated P deficiency in rice (Oryza sativa). Results P deficiency decreased ABA accumulation rapidly (within 1 h) in the roots. Exogenous ABA negatively regulated root and shoot soluble P contents by decreasing pectin content, inhibiting P deficiency-induced increases in pectin methylesterase activity and expression of the phosphate transporter gene-OsPT6, thereby decreasing the re-utilization of P from the cell wall and its translocation to the shoot. Moreover, neither the nitric oxide (NO) donor sodium nitroprusside nor ethylene precursor 1-aminocyclopropane-1-carboxylic acid had any effect on ABA accumulation, and application of ABA or the ABA inhibitor fluridone also had no effect on NO production and ethylene emission. Conclusions Under P deficiency, NO levels increase as quickly as ABA levels decrease, to inhibit both the ABA-induced reduction of pectin contents for the re-utilization of cell wall P and the ABA-induced down-regulation of OsPT6 for the translocation of P from roots to shoots. Overall, our results provide novel information indicating that the reduction of ABA under P deficiency is a very important pathway in the re-utilization of cell wall P in rice under P-deficient conditions, which should be a very effective mechanism for plant survival under P deficiency stress for common agronomic practice.