Fractionation techniques to increase plant proteome coverage: Combining separation in parallel at the protein and the peptide level

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Abstract

Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.

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Černý, M., Berka, M., & Habánová, H. (2019). Fractionation techniques to increase plant proteome coverage: Combining separation in parallel at the protein and the peptide level. In Methods in Molecular Biology (Vol. 1871, pp. 93–105). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8814-3_6

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