TOTAL PHENOLIC, FLAVONOID, TANNIN CONTENTS AND ANTIOXIDANT PROPERTIES OF PLEUROTUS OSTREATUS AND PLEUROTUS CITRINOPILEATUS CULTIVATED ON VARIOUS SAWDUST

Abstract

In this study, the possibility of using of chestnut (Castanea sativa) sawdust in Oyster mushroom (Pleurotus ostretatus and Pleurotus citrinopileatus) cultivation was investigated. Additionally; Pleurotus ostreatus cultivation on the substrates which was mixed of chestnut with black poplar (Populus nigra) and oriental spruce (Picea orientalis) was performed. Bioactive properties of these mushroom and their growth mediums were also examined. After a successful harvest, total phenolic, flavonoid, condensed tannin contents and antioxidant properties of mushrooms’ methanolic extracts were determined. Same analyses were also performed for mushrooms substrates. The highest yield and biological efficiency was observed in P. ostreatus cultivated on 100% Castanea sativa substrate. The highest total phenolic content (2.529±0.010 mg GAE/g) was found in P. citrinopileatus cultivated on C. sativa sawdust and its substrate medium. Total flavonoid could not determine any mushroom. The highest total condensed tannin (3.691±0.011 CE mg/g) content was observed in P. ostreatus cultivated on C. sativa sawdust and its substrate medium; the highest ferric reducing antioxidant power (11.761±0.020 µmol FeSO4.7H2O/g) was seen in P. ostreatus mushroom cultivated on 50% P. orientalis + 50% C. sativa and 100% C. sativa substrate medium. The highest free radical scavenging activity of DPPH was in P. citrinopileatus and 100% C. sativa sawdust and 100% C. sativa substrate medium