The prefemoral efferent lymphatics of four sheep persistently infected with a non cytopathic (NCP) isolate of border disease virus (BDV) were cannulated. Recovered lymphocytes were examined for the presence of virus by an immunocytochemical technique employing a pool of monoclonal antibodies which recognise the 120K non-structural polypeptide of NCP BDV. The results revealed that 9.5% of the lymphocytes carried virus antigen. Lymphocytes from two of the sheep were studied by in situ hybridisation using a viral antisense RNA probe complementary to the region of the BDV genome coding for the 120K polypeptide. This showed that 70-80% of the cells were infected, confirming the greater sensitivity of the in situ hybridisation technique.
CITATION STYLE
Entrican, G., Flack, A., Hopkins, J., MacLean, M., & Nettleton, P. F. (1991). Detection of border disease virus in sheep efferent lymphocytes by immunocytochemical and in situ hybridisation techniques. Archives of Virology. Supplementum, 3, 175–180. https://doi.org/10.1007/978-3-7091-9153-8_21
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