Genetic variability in labeo rohita, Catla catla and their hybrid (Labeo rohita ♂ × Catla catla ♀) populations employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) assays

Abstract

The present study was conducted to estimate genetic diversity of hybrid produced by two major carps (Labeo rohita and Catla catla) using DNA fingerprinting RAPD-ISSRs assay. A total of 65 RAPD-ISSR combinations were studied to check polymorphism among the accessions. 866 loci were amplified with an average of 13.32 loci per primer and 45.26% were found informative. Primers: DDA31-ISSR1 and DDA32-ISSR2 were most informative producing 12 polymorphic bands each. A similarity of 82% and 63% was exhibited by C. catla and their hybrids and L. rohita and their hybrids, respectively. Similarity analysis resulted 92% similarity among L. rohita parents' group and 84% similarity for their hybrids. Cluster analysis divided all accessions into two clusters: A and B; placing L. rohita in-group A, while B cluster was divided into two Sub groups B1 and B2 having C. catla and hybrids, respectively. The low level of genetic diversity, clearly signposted the alarming need of diverse parental populations for fish breeding programs and saving hybrid gene pool losses. It is the first report molecular program for the selection of major carps for breeding of fish in seed hatcheries.