The formation of granulomas is associated with the resolution of Q fever, a zoonosis due to Coxiella burnetii; however the molecular mechanisms of granuloma formation remain poorly understood. We generated human granulomas with peripheral blood mononuclear cells and beads coated with C. burnetii, using BCG extracts as controls. A microarray analysis showed dramatic changes in gene expression in granuloma cells of which more than 50% were commonly modulated genes in response to C. burnetii and BCG. They included M1-related genes and genes related to chemotaxis. The inhibition of the chemokines, CCL2 and CCL5, directly interfered with granuloma formation. C. burnetii granulomas also expressed a specific transcriptional profile that was essentially enriched in genes associated with type I interferon response. Our results showed that granuloma formation is associated with a core of transcriptional response based on inflammatory genes. The specific granulomatous response to C. burnetii is characterized by the activation of type 1 interferon pathway.
CITATION STYLE
Faugaret, D., Ben-Amara, A., Alingrin, J., Daumas, A., Delaby, A., Lépolard, C., … Mége, J. L. (2014). Granulomatous response to Coxiella burnetii, the agent of Q fever: The lessons from gene expression analysis. Frontiers in Cellular and Infection Microbiology, 4(NOV). https://doi.org/10.3389/fcimb.2014.00172
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