This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
CITATION STYLE
van’t Hof, R. J. (2019). The calvarial injection assay. In Methods in Molecular Biology (Vol. 1914, pp. 361–368). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8997-3_21
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