Use of computer system for cell hybridisation in biotechnology and medicine

Abstract

An outline of issues relating to the contemporary application of monoclone antibodies and techniques for obtaining hybrids has been presented in the study. The results of research concerning modifications of Zimmerman's methods have been presented. They prove a higher efficacy and selectivity of the solution proposed. An electroporation system with changed geometry of electrodes and current parameters has been presented. The procedure is controlled entirely by a microprocessor from the stage of technological parameters' control in the incubators of initial cells to the creation of a determined mixture of cells in a mixer, then division into portions, pumping the mixture into a hybridisation chamber and, following hybridisation, passing the mixture into separate sections of the incubator. The hybridisation system was modified. A transparent water coat was constructed and connected to a thermostat, on which a transparent hybridisation chamber was installed. Lighting from underneath and gap lighting of the chamber enable continuous observation of suspended cells by means of a microscope lens which is connected by a picture channel to a computer. The software analyses the picture in terms of hybrid selection. The marked cells are planimetrically analysed during the programmed duration. When the morphometric criteria are met, the suspended cells are pumped over to separate sections of the incubator, where selective breeding of hybrids is carried out. The selection of hybrids takes place in electroosmosis gradient under morphometric control of cells in microcapillary systems. © 2008 Springer-Verlag Berlin Heidelberg.